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Refractometry: Theory. The speed of light in a vacuum is always the same, but.
The ratio of the speed of light in a vacuum to the speed of light in another. Figure 1. Light crossing. In the. case shown, the speed of light in medium A is greater than the speed. B. Whenever light changes speed as it crosses a boundary. Figure 1). (In the special case of the light traveling.
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The relationship between light's speed in the two mediums. A and v. B), the angles. A). and refraction (q. B). and the refractive indexes of the two mediums (n. A. and n. B) is shown below: Thus, it is not necessary to measure the speed of light.
Instead, by. measuring the angle of refraction, and knowing the index of refraction. Nearly all refractometers. Figure 2. Cross section.
Abbe refractometer. The sample. thickness has been exaggerated for clarity.
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In the Abbe' refractometer the liquid sample is sandwiched. Figure 2). The refracting prism is made of a glass with a high refractive. A. light source is projected through the illuminating prism, the bottom surface. Inspection of Figure 2 shows that light traveling from. A to point B will have the largest angle of incidence (qi). All other rays of light entering the refracting prism.
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C. Thus, a detector placed on the back. Samples with different refractive indexes will produce.
Equation 2 above and recall that the. By appropriately calibrating the scale, the. In an actual Abbe' refractometer there is not a detector.
A guide to using the refractometer -- Background and Theory. Refractometry: Introduction. Theory. Anatomy of a Refractometer. SOP: Bausch & Lomb Abbe-3L Refractometer.
It is also possible to design a. These types of refractometers are often.
In most liquids and solids the speed of light, and hence. This variation. is referred to as dispersion, and it is what causes white. Shorter wavelengths. Thus, for the most accurate.
The most widely. used wavelength of light for refractometry is the sodium D line at 5. If white light were used in the simple Abbe' refractometer. Figure 2, dispersion would result in the light and dark.
The resulting "fuzziness" of the borderline would make precise. However, many Abbe' refractometers are able to operate. These compensating.
D line. As mentioned earlier, the speed of light in a substance. Since the density of a liquid usually decreases. Thus, the. index of refraction normally decreases as the temperature increases. Table 1). For many organic liquids the index of refraction.
C increase in temperature. However for water the variation is only about - 0. C. Many refractometers are equipped with a thermometer. Most of the refractive index measurements reported.